Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.
Identifieur interne : 000E89 ( Main/Exploration ); précédent : 000E88; suivant : 000E90Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.
Auteurs : Xiaoqiu Wu [Suède] ; Hans Jörnvall ; Kurt D. Berndt ; Udo OppermannSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- ARN de transfert (génétique), ARN de transfert (métabolisme), ARN messager (biosynthèse), ARN messager (génétique), Biosynthèse des protéines (génétique), Codon (MeSH), Dichroïsme circulaire (MeSH), Escherichia coli (génétique), Escherichia coli (métabolisme), Fusion artificielle de gènes (MeSH), Gènes d'archée (MeSH), Methanobacterium (génétique), Protéines d'archée (biosynthèse), Protéines d'archée (composition chimique), Protéines d'archée (génétique), Protéines de fusion recombinantes (biosynthèse), Protéines de fusion recombinantes (composition chimique), Protéines de fusion recombinantes (génétique), Régulation de l'expression des gènes bactériens (génétique), Spectrométrie de masse ESI (méthodes), Stabilité de l'ARN (génétique), Structure secondaire des protéines (MeSH), Sulfolobus (génétique).
- MESH :
- biosynthèse : ARN messager, Protéines d'archée, Protéines de fusion recombinantes.
- composition chimique : Protéines d'archée, Protéines de fusion recombinantes.
- génétique : ARN de transfert, ARN messager, Biosynthèse des protéines, Escherichia coli, Methanobacterium, Protéines d'archée, Protéines de fusion recombinantes, Régulation de l'expression des gènes bactériens, Stabilité de l'ARN, Sulfolobus.
- métabolisme : ARN de transfert, Escherichia coli.
- méthodes : Spectrométrie de masse ESI.
- Codon, Dichroïsme circulaire, Fusion artificielle de gènes, Gènes d'archée, Structure secondaire des protéines.
English descriptors
- KwdEn :
- Archaeal Proteins (biosynthesis), Archaeal Proteins (chemistry), Archaeal Proteins (genetics), Artificial Gene Fusion (MeSH), Circular Dichroism (MeSH), Codon (MeSH), Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression Regulation, Bacterial (genetics), Genes, Archaeal (MeSH), Methanobacterium (genetics), Protein Biosynthesis (genetics), Protein Structure, Secondary (MeSH), RNA Stability (genetics), RNA, Messenger (biosynthesis), RNA, Messenger (genetics), RNA, Transfer (genetics), RNA, Transfer (metabolism), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (genetics), Spectrometry, Mass, Electrospray Ionization (methods), Sulfolobus (genetics).
- MESH :
- chemical , biosynthesis : Archaeal Proteins, RNA, Messenger, Recombinant Fusion Proteins.
- chemical , chemistry : Archaeal Proteins, Recombinant Fusion Proteins.
- chemical , genetics : Archaeal Proteins, RNA, Messenger, RNA, Transfer, Recombinant Fusion Proteins.
- genetics : Escherichia coli, Gene Expression Regulation, Bacterial, Methanobacterium, Protein Biosynthesis, RNA Stability, Sulfolobus.
- metabolism : Escherichia coli, RNA, Transfer.
- methods : Spectrometry, Mass, Electrospray Ionization.
- Artificial Gene Fusion, Circular Dichroism, Codon, Genes, Archaeal, Protein Structure, Secondary.
Abstract
Expression patterns in Escherichia coli of two small archaeal proteins with a natural content of about 30% rare codons were analyzed. The proteins, a histone-like protein from Sulfolobus shibatae (Ssh10), and a glutaredoxin-like protein from Methanobacterium thermoautotrophicum (mtGrx), were produced with expression plasmids encoding wild-type genes, codon-optimized synthetic, and GST-fusion genes. These constructs were expressed in BL21 (DE3), its LysS derivative, and modified strains carrying copies for rare codon tRNAs or deletions in the RNAseE gene. Both Ssh10 and mtGrx expression levels were constitutively high in BL21(DE3) and its derivatives, with the exception of the LysS phenotype, which prevented high level expression of the Ssh10 wild-type gene. Surprisingly, a codon-optimized mtGrx gene construct displayed undetectable levels of protein production. The translational block observed with the synthetic mtGrx gene could be circumvented by using a synthetic mtGrx-glutathione S-transferase (GST) fusion construct or by in vitro translation. Taken together, the results underscore the importance of mRNA levels and RNA stability, but not necessarily tRNA abundance for efficient heterologous protein production in E. coli.
DOI: 10.1016/j.bbrc.2003.11.091
PubMed: 14672702
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.</title><author><name sortKey="Wu, Xiaoqiu" sort="Wu, Xiaoqiu" uniqKey="Wu X" first="Xiaoqiu" last="Wu">Xiaoqiu Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE-171 77, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE-171 77</wicri:regionArea><wicri:noRegion>Stockholm SE-171 77</wicri:noRegion></affiliation></author><author><name sortKey="Jornvall, Hans" sort="Jornvall, Hans" uniqKey="Jornvall H" first="Hans" last="Jörnvall">Hans Jörnvall</name></author><author><name sortKey="Berndt, Kurt D" sort="Berndt, Kurt D" uniqKey="Berndt K" first="Kurt D" last="Berndt">Kurt D. Berndt</name></author><author><name sortKey="Oppermann, Udo" sort="Oppermann, Udo" uniqKey="Oppermann U" first="Udo" last="Oppermann">Udo Oppermann</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14672702</idno><idno type="pmid">14672702</idno><idno type="doi">10.1016/j.bbrc.2003.11.091</idno><idno type="wicri:Area/Main/Corpus">000E97</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E97</idno><idno type="wicri:Area/Main/Curation">000E97</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E97</idno><idno type="wicri:Area/Main/Exploration">000E97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.</title><author><name sortKey="Wu, Xiaoqiu" sort="Wu, Xiaoqiu" uniqKey="Wu X" first="Xiaoqiu" last="Wu">Xiaoqiu Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE-171 77, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE-171 77</wicri:regionArea><wicri:noRegion>Stockholm SE-171 77</wicri:noRegion></affiliation></author><author><name sortKey="Jornvall, Hans" sort="Jornvall, Hans" uniqKey="Jornvall H" first="Hans" last="Jörnvall">Hans Jörnvall</name></author><author><name sortKey="Berndt, Kurt D" sort="Berndt, Kurt D" uniqKey="Berndt K" first="Kurt D" last="Berndt">Kurt D. Berndt</name></author><author><name sortKey="Oppermann, Udo" sort="Oppermann, Udo" uniqKey="Oppermann U" first="Udo" last="Oppermann">Udo Oppermann</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Archaeal Proteins (biosynthesis)</term><term>Archaeal Proteins (chemistry)</term><term>Archaeal Proteins (genetics)</term><term>Artificial Gene Fusion (MeSH)</term><term>Circular Dichroism (MeSH)</term><term>Codon (MeSH)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Gene Expression Regulation, Bacterial (genetics)</term><term>Genes, Archaeal (MeSH)</term><term>Methanobacterium (genetics)</term><term>Protein Biosynthesis (genetics)</term><term>Protein Structure, Secondary (MeSH)</term><term>RNA Stability (genetics)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (genetics)</term><term>RNA, Transfer (genetics)</term><term>RNA, Transfer (metabolism)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (chemistry)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term><term>Sulfolobus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN de transfert (génétique)</term><term>ARN de transfert (métabolisme)</term><term>ARN messager (biosynthèse)</term><term>ARN messager (génétique)</term><term>Biosynthèse des protéines (génétique)</term><term>Codon (MeSH)</term><term>Dichroïsme circulaire (MeSH)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Fusion artificielle de gènes (MeSH)</term><term>Gènes d'archée (MeSH)</term><term>Methanobacterium (génétique)</term><term>Protéines d'archée (biosynthèse)</term><term>Protéines d'archée (composition chimique)</term><term>Protéines d'archée (génétique)</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de fusion recombinantes (composition chimique)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Régulation de l'expression des gènes bactériens (génétique)</term><term>Spectrométrie de masse ESI (méthodes)</term><term>Stabilité de l'ARN (génétique)</term><term>Structure secondaire des protéines (MeSH)</term><term>Sulfolobus (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Archaeal Proteins</term><term>RNA, Messenger</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Archaeal Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Archaeal Proteins</term><term>RNA, Messenger</term><term>RNA, Transfer</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Protéines d'archée</term><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines d'archée</term><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Gene Expression Regulation, Bacterial</term><term>Methanobacterium</term><term>Protein Biosynthesis</term><term>RNA Stability</term><term>Sulfolobus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN de transfert</term><term>ARN messager</term><term>Biosynthèse des protéines</term><term>Escherichia coli</term><term>Methanobacterium</term><term>Protéines d'archée</term><term>Protéines de fusion recombinantes</term><term>Régulation de l'expression des gènes bactériens</term><term>Stabilité de l'ARN</term><term>Sulfolobus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>RNA, Transfer</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN de transfert</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Spectrométrie de masse ESI</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Artificial Gene Fusion</term><term>Circular Dichroism</term><term>Codon</term><term>Genes, Archaeal</term><term>Protein Structure, Secondary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Codon</term><term>Dichroïsme circulaire</term><term>Fusion artificielle de gènes</term><term>Gènes d'archée</term><term>Structure secondaire des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Expression patterns in Escherichia coli of two small archaeal proteins with a natural content of about 30% rare codons were analyzed. The proteins, a histone-like protein from Sulfolobus shibatae (Ssh10), and a glutaredoxin-like protein from Methanobacterium thermoautotrophicum (mtGrx), were produced with expression plasmids encoding wild-type genes, codon-optimized synthetic, and GST-fusion genes. These constructs were expressed in BL21 (DE3), its LysS derivative, and modified strains carrying copies for rare codon tRNAs or deletions in the RNAseE gene. Both Ssh10 and mtGrx expression levels were constitutively high in BL21(DE3) and its derivatives, with the exception of the LysS phenotype, which prevented high level expression of the Ssh10 wild-type gene. Surprisingly, a codon-optimized mtGrx gene construct displayed undetectable levels of protein production. The translational block observed with the synthetic mtGrx gene could be circumvented by using a synthetic mtGrx-glutathione S-transferase (GST) fusion construct or by in vitro translation. Taken together, the results underscore the importance of mRNA levels and RNA stability, but not necessarily tRNA abundance for efficient heterologous protein production in E. coli.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14672702</PMID><DateCompleted><Year>2004</Year><Month>02</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>313</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jan</Month><Day>02</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.</ArticleTitle><Pagination><MedlinePgn>89-96</MedlinePgn></Pagination><Abstract><AbstractText>Expression patterns in Escherichia coli of two small archaeal proteins with a natural content of about 30% rare codons were analyzed. The proteins, a histone-like protein from Sulfolobus shibatae (Ssh10), and a glutaredoxin-like protein from Methanobacterium thermoautotrophicum (mtGrx), were produced with expression plasmids encoding wild-type genes, codon-optimized synthetic, and GST-fusion genes. These constructs were expressed in BL21 (DE3), its LysS derivative, and modified strains carrying copies for rare codon tRNAs or deletions in the RNAseE gene. Both Ssh10 and mtGrx expression levels were constitutively high in BL21(DE3) and its derivatives, with the exception of the LysS phenotype, which prevented high level expression of the Ssh10 wild-type gene. Surprisingly, a codon-optimized mtGrx gene construct displayed undetectable levels of protein production. The translational block observed with the synthetic mtGrx gene could be circumvented by using a synthetic mtGrx-glutathione S-transferase (GST) fusion construct or by in vitro translation. Taken together, the results underscore the importance of mRNA levels and RNA stability, but not necessarily tRNA abundance for efficient heterologous protein production in E. coli.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiaoqiu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE-171 77, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jörnvall</LastName><ForeName>Hans</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Berndt</LastName><ForeName>Kurt D</ForeName><Initials>KD</Initials></Author><Author ValidYN="Y"><LastName>Oppermann</LastName><ForeName>Udo</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019843">Archaeal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003062">Codon</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9014-25-9</RegistryNumber><NameOfSubstance UI="D012343">RNA, Transfer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019843" MajorTopicYN="N">Archaeal Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019677" MajorTopicYN="N">Artificial Gene Fusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002942" MajorTopicYN="N">Circular Dichroism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003062" MajorTopicYN="Y">Codon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019766" MajorTopicYN="N">Genes, Archaeal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017014" MajorTopicYN="N">Methanobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017433" MajorTopicYN="N">Protein Structure, Secondary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020871" MajorTopicYN="N">RNA Stability</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012343" MajorTopicYN="N">RNA, Transfer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016944" MajorTopicYN="N">Sulfolobus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>12</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>2</Month><Day>5</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>12</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14672702</ArticleId><ArticleId IdType="pii">S0006291X03024653</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2003.11.091</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Berndt, Kurt D" sort="Berndt, Kurt D" uniqKey="Berndt K" first="Kurt D" last="Berndt">Kurt D. Berndt</name><name sortKey="Jornvall, Hans" sort="Jornvall, Hans" uniqKey="Jornvall H" first="Hans" last="Jörnvall">Hans Jörnvall</name><name sortKey="Oppermann, Udo" sort="Oppermann, Udo" uniqKey="Oppermann U" first="Udo" last="Oppermann">Udo Oppermann</name></noCountry><country name="Suède"><noRegion><name sortKey="Wu, Xiaoqiu" sort="Wu, Xiaoqiu" uniqKey="Wu X" first="Xiaoqiu" last="Wu">Xiaoqiu Wu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E89 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E89 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GlutaredoxinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:14672702
|texte= Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:14672702" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GlutaredoxinV1
| This area was generated with Dilib version V0.6.37. |  |